Evaluation of locomotor activity and eugenol toxicity test using adult zebrafish (danio rerio).

Authors

DOI:

https://doi.org/10.24979/ambiente.v1i1.931

Keywords:

Eugenol, Locomotor system, Zebrafish, Locomotion

Abstract

Eugenol is the major component of the essential oil of cloves (Syzygium aromaticum). It has an effect on the central nervous system in mice and rats. The present study aimed to evaluate the locomotor activity and acute toxicity of eugenol using adult zebrafish. For the open field test, zebrafish received doses of eugenol (4, 20 and 40 mg / kg); DMSO 3% and DZP 40mg / kg. After 30 min, the animals were added to Petri dishes, and locomotor activity was analyzed by counting the number of CL (5 min). For toxicity assessment, the animals received the same treatments and after 96 hours of analysis, the values ​​obtained with the number of ZFa killed were subjected to statistical analysis to estimate DL to kill 50%. Eugenol altered the zebrafish's locomotor system and was not toxic until 96 hours of analysis. The absence of toxicity and the alteration in the zebrafish locomotor system shows the non-clinical safety of eugenol and allows us to continue testing and investigate possible anxiolytic and anticonvulsant effects.

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Author Biographies

  • Cecilia Guimaraes Lemos, State University of Ceará
      Undergraduate student in Chemistry.
  • Jean Parcelli Costa do Vale, State University of Vale do Acaraú

    Professor at Vale do Acaraú State University, Sobral, Cear. Center for Exact Sciences and Technology

  • Maria Kueirislene Amancio Ferreira, State University of Ceará

    PhD student in Natural Sciences.

  • Antonio Wlisses da Silva, State University of Ceará

    PhD student in Biotechnology of Natural Resources - Renorbio.

  • Jane Eire Silva Alencar de Menezes, State University of Ceará

    Professor of the Postgraduate Program in Natural Sciences, State University of Ceará, Center for Science and Technology.

  • Helcio Silva dos Santos , State University of Vale do Acaraú

    Degree in Chemistry with a Master's and Ph.D. in Organic Chemistry from the Federal University of Ceará. Postdoctoral in synthesis of chalcones with antimicrobial potential carried out at the Federal University of Ceará (2018-2019). He is currently Associate Professor at the State University of Vale do Acaraú-UVA, permanent professor at the Postgraduate Programs Northeast Biotechnology Network (RENORBIO-Nucleadora UECE), Postgraduate Studies in Biological Chemistry at the Regional University of Cariri-URCA and Postgraduate Studies in Natural Sciences of the State University of Ceará-UECE. He published 117 articles in specialized international and national journals, which attracted, until July 2021, 613 citations resulting in a factor h = 12. He was Coordinator (03 / 2010-03-2014) and vice-coordinator (03 / 2008-02 / 2010) of the Chemistry course at the State University of Vale do Acaraú. Has experience in Organic Chemistry, focusing on Natural Products Chemistry and synthesis of chalcones, acting on the following subjects: isolation, purification and structural elucidation of natural products, synthesis of chalcones, hydrazones, analysis of secondary metabolites and synthetic compounds using Raman spectroscopy, antimicrobial and anxiolytic activities using Zebrafish (Danio rerio) of natural and synthetic compounds.

References

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Published

2021-08-31

Issue

2021: Dossier: 1st Environment and Energy Symposium (SiMAE)

Section

Dossiers

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Copyright (c) 2021 Cecília Guimarães Lemos, Jean Parcelli Costa do Vale, Maria Kueirislene Amâncio Ferreira, Antônio Wlisses da Silva, Jane Eire Silva Alencar de Menezes

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How to Cite

Evaluation of locomotor activity and eugenol toxicity test using adult zebrafish (danio rerio). Ambiente: Gestão e Desenvolvimento, [S. l.], v. 1, n. 1, 2021. DOI: 10.24979/ambiente.v1i1.931. Disponível em: https://periodicos.uerr.edu.br/index.php/ambiente/article/view/931. Acesso em: 12 mar. 2026.

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